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Recombinant Human Growth Factor β1, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Temporal analysis of the BMSC paracrine profile on different scaffolds. (A) Confocal microscopy images from Live/Dead fluorescence staining of BMSCs encapsulated within the PCL/HAp-GelMA/BMSCs scaffold after 1, 3, 5, and 14 d of 3D culture (live cells, green; dead cells, red). (B) The concentrations of key paracrine factors <t>(TGF-β,</t> <t>PGE2,</t> VEGF, HGF, and BMP-2) from BMSCs cultured in different scaffolds, quantified from culture supernatants at day 3 and day 7. (C) Corresponding relative mRNA expression levels of TGFB1, PTGS2, VEGFA, HGF, and BMP-2 in BMSCs at day 3 and day 7, as determined by qPCR analysis. Data are presented as mean ± SD (n = 3) *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001; ns: not significant.
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MedChemExpress growth factor β1
Temporal analysis of the BMSC paracrine profile on different scaffolds. (A) Confocal microscopy images from Live/Dead fluorescence staining of BMSCs encapsulated within the PCL/HAp-GelMA/BMSCs scaffold after 1, 3, 5, and 14 d of 3D culture (live cells, green; dead cells, red). (B) The concentrations of key paracrine factors <t>(TGF-β,</t> <t>PGE2,</t> VEGF, HGF, and BMP-2) from BMSCs cultured in different scaffolds, quantified from culture supernatants at day 3 and day 7. (C) Corresponding relative mRNA expression levels of TGFB1, PTGS2, VEGFA, HGF, and BMP-2 in BMSCs at day 3 and day 7, as determined by qPCR analysis. Data are presented as mean ± SD (n = 3) *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001; ns: not significant.
Growth Factor β1, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Temporal analysis of the BMSC paracrine profile on different scaffolds. (A) Confocal microscopy images from Live/Dead fluorescence staining of BMSCs encapsulated within the PCL/HAp-GelMA/BMSCs scaffold after 1, 3, 5, and 14 d of 3D culture (live cells, green; dead cells, red). (B) The concentrations of key paracrine factors <t>(TGF-β,</t> <t>PGE2,</t> VEGF, HGF, and BMP-2) from BMSCs cultured in different scaffolds, quantified from culture supernatants at day 3 and day 7. (C) Corresponding relative mRNA expression levels of TGFB1, PTGS2, VEGFA, HGF, and BMP-2 in BMSCs at day 3 and day 7, as determined by qPCR analysis. Data are presented as mean ± SD (n = 3) *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001; ns: not significant.
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Temporal analysis of the BMSC paracrine profile on different scaffolds. (A) Confocal microscopy images from Live/Dead fluorescence staining of BMSCs encapsulated within the PCL/HAp-GelMA/BMSCs scaffold after 1, 3, 5, and 14 d of 3D culture (live cells, green; dead cells, red). (B) The concentrations of key paracrine factors <t>(TGF-β,</t> <t>PGE2,</t> VEGF, HGF, and BMP-2) from BMSCs cultured in different scaffolds, quantified from culture supernatants at day 3 and day 7. (C) Corresponding relative mRNA expression levels of TGFB1, PTGS2, VEGFA, HGF, and BMP-2 in BMSCs at day 3 and day 7, as determined by qPCR analysis. Data are presented as mean ± SD (n = 3) *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001; ns: not significant.
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Affinity Biosciences transforming growth factor β1 tgf β1 antibody
In vivo therapeutic efficacy. (A) TNF-α, (B) IL-1β, (C) SOD, and (D) MDA levels in renal tissues after different drug treatments. (E) Immunofluorescence detection of CD86 expression in the glomeruli. Scale bars: 100 μm. (F) Immunofluorescence detection of 8-OHdG expression in the kidney. Scale bars: 300 μm. (G) Immunofluorescence detection of PDGF-BB <t>and</t> <t>TGF-β1</t> expression in the glomeruli. Scale bars: 100 μm. (H-K) Fluorescence statistical graph of CD86, 8-OHdG, PDGF-BB, and TGF-β1. ∗ P < 0.05 and ∗∗ P < 0.01.
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OriGene latent transforming growth factor β1
In vivo therapeutic efficacy. (A) TNF-α, (B) IL-1β, (C) SOD, and (D) MDA levels in renal tissues after different drug treatments. (E) Immunofluorescence detection of CD86 expression in the glomeruli. Scale bars: 100 μm. (F) Immunofluorescence detection of 8-OHdG expression in the kidney. Scale bars: 300 μm. (G) Immunofluorescence detection of PDGF-BB <t>and</t> <t>TGF-β1</t> expression in the glomeruli. Scale bars: 100 μm. (H-K) Fluorescence statistical graph of CD86, 8-OHdG, PDGF-BB, and TGF-β1. ∗ P < 0.05 and ∗∗ P < 0.01.
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Temporal analysis of the BMSC paracrine profile on different scaffolds. (A) Confocal microscopy images from Live/Dead fluorescence staining of BMSCs encapsulated within the PCL/HAp-GelMA/BMSCs scaffold after 1, 3, 5, and 14 d of 3D culture (live cells, green; dead cells, red). (B) The concentrations of key paracrine factors (TGF-β, PGE2, VEGF, HGF, and BMP-2) from BMSCs cultured in different scaffolds, quantified from culture supernatants at day 3 and day 7. (C) Corresponding relative mRNA expression levels of TGFB1, PTGS2, VEGFA, HGF, and BMP-2 in BMSCs at day 3 and day 7, as determined by qPCR analysis. Data are presented as mean ± SD (n = 3) *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001; ns: not significant.

Journal: Bioactive Materials

Article Title: Mesenchymal stromal cells-loaded 3D radially aligned composite scaffold with potentiated paracrine signaling for sequential bone regeneration

doi: 10.1016/j.bioactmat.2026.02.059

Figure Lengend Snippet: Temporal analysis of the BMSC paracrine profile on different scaffolds. (A) Confocal microscopy images from Live/Dead fluorescence staining of BMSCs encapsulated within the PCL/HAp-GelMA/BMSCs scaffold after 1, 3, 5, and 14 d of 3D culture (live cells, green; dead cells, red). (B) The concentrations of key paracrine factors (TGF-β, PGE2, VEGF, HGF, and BMP-2) from BMSCs cultured in different scaffolds, quantified from culture supernatants at day 3 and day 7. (C) Corresponding relative mRNA expression levels of TGFB1, PTGS2, VEGFA, HGF, and BMP-2 in BMSCs at day 3 and day 7, as determined by qPCR analysis. Data are presented as mean ± SD (n = 3) *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001; ns: not significant.

Article Snippet: ELISA kits for PGE2 (Cat. No. E-EL-0034), TGF-β (Cat. No. E-EL-0162), VEGF (Cat. No. E-EL-R2603), and HGF (Cat. No. E-EL-R0496) were purchased from Elabscience (Wuhan, China).

Techniques: Confocal Microscopy, Fluorescence, Staining, Cell Culture, Expressing

In vivo therapeutic efficacy. (A) TNF-α, (B) IL-1β, (C) SOD, and (D) MDA levels in renal tissues after different drug treatments. (E) Immunofluorescence detection of CD86 expression in the glomeruli. Scale bars: 100 μm. (F) Immunofluorescence detection of 8-OHdG expression in the kidney. Scale bars: 300 μm. (G) Immunofluorescence detection of PDGF-BB and TGF-β1 expression in the glomeruli. Scale bars: 100 μm. (H-K) Fluorescence statistical graph of CD86, 8-OHdG, PDGF-BB, and TGF-β1. ∗ P < 0.05 and ∗∗ P < 0.01.

Journal: Materials Today Bio

Article Title: Biomimetic self-assembled nanosoldiers of paotianxiong polysaccharides and α-lipoic acid: Highly specific targeted therapy for mesangial proliferative glomerulonephritis

doi: 10.1016/j.mtbio.2026.103313

Figure Lengend Snippet: In vivo therapeutic efficacy. (A) TNF-α, (B) IL-1β, (C) SOD, and (D) MDA levels in renal tissues after different drug treatments. (E) Immunofluorescence detection of CD86 expression in the glomeruli. Scale bars: 100 μm. (F) Immunofluorescence detection of 8-OHdG expression in the kidney. Scale bars: 300 μm. (G) Immunofluorescence detection of PDGF-BB and TGF-β1 expression in the glomeruli. Scale bars: 100 μm. (H-K) Fluorescence statistical graph of CD86, 8-OHdG, PDGF-BB, and TGF-β1. ∗ P < 0.05 and ∗∗ P < 0.01.

Article Snippet: Transforming growth factor-β1 (TGF-β1) antibody was obtained from Jiangsu Affinity Biosciences Co., Ltd. (Changzhou, China).

Techniques: In Vivo, Drug discovery, Immunofluorescence, Expressing, Fluorescence